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The authors thank Thierry Dumas for helpful discussion and Karel Kylli for technical assistance.
L.G. has been working on cytosolic Ca^2+^ signaling for many years and the experimenters of the Laboratory of the Phosphoinositides are most indebted to him for having demonstrated that Ca^2+^ release from the endoplasmic reticulum induces a small decrease of cytosolic Ca^2+^ in the absence of IP~3~R activation in H9C2 cells (1 and 2). E.C. was the first to show that the IP~3~R can be activated by IP~3~ in the cytosol in cultured smooth muscle cells and heart cells. He went on to study Ca^2+^ dynamics in Purkinje cells in Xenopus oocytes, cardiac and smooth muscle cells. T.H.G. was also one of the first to monitor transmembrane Ca^2+^ influxes evoked by excitation. The Laboratory of the Phosphoinositides is grateful to Jean-Claude Duby for his exceptional work on the ultrastructure of giant synapses in the 1990s. He is now a professor at the Electron Microscopy (EM) Department of the Institut Curie in Paris. K.D. holds the Chair of Cellular and Molecular Ca^2+^ Signalling in Cardiac Diseases and is a member of the Cardiovascular Research Institute Maastricht, an Interuniversity Cardiology Institute. Together with his collaborators, he developed the cardiomyocyte microfluorimetric technique for measuring both cardiac sarcoplasmic reticulum (SR) Ca^2+^ stores and cytosolic Ca^2+^ ([@b17]). B.M. was a post-doctoral researcher of Louis Gachon (LGBio) in Ca^2+^ signaling at the Pasteur Institute, who, together with J.D. Bezard, showed that a transient sustained increase in cytos
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